bacterial suspension  (ATCC)

Journal: Antibiotics

Article Title: β,β-Dimethylacrylalkannin Restores Colistin Efficacy Against mcr - and TCS-Mediated Resistant Gram-Negative Bacteria via Membrane Disturbance

doi: 10.3390/antibiotics15010003

Figure Lengend Snippet: β,β-Dim targets the phospholipids and disrupts cytoplasmic membrane biofunction. ( A ) Effects of β, β-Dim on membrane permeability in E. coli spheroplasts. ( B ) Intracellular ATP level in E. coli . ( C ) Influence of PE, PG, and CL on the antibacterial activity of β, β-Dim against S. aureus ATCC 29213. ( D ) Binding affinity of β, β-Dim for CL measured by ITC assays. ( E ) Intracellular β,β-Dim levels in E. coli BW25113, E. coli BW25113- mcr-1 , and E. coli BW25113- pmrB R93P following treatment with varying concentrations of colistin. Data represent three biological replicates. COL: colistin. Unit: μg/mL. ns, no significant difference. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The S. aureus ATCC 29213 bacterial suspension (OD600 = 0.5) was incubated with fluorescent probe propidium iodide (PI, Cat No. C1008S, Beyotime, Shanghai, China, final concentration 10 μM) for 30 min. Then, β,β-Dim (0–32 μg/mL) was added to the PI-labeled suspension in a black 96-well plate and incubated for 1 h. Fluorescence intensity was measured at an excitation/emission wavelength of 535/615 nm.

Techniques: Membrane, Permeability, Activity Assay, Binding Assay

Journal: Microorganisms

Article Title: Antimicrobial Activities of Propolis Nanoparticles in Combination with Ampicillin Sodium Against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/microorganisms13081844

Figure Lengend Snippet: Synergistic effect of AS with PNPs. ( A ) Schematic checkerboard of MRSA ATCC 43300 growth inhibition with varying concentrations of PNPs and AS. ( B ) Schematic checkerboard of MRSA ATCC 33591 growth inhibition with varying concentrations of PNPs and AS. ( C ) Schematic checkerboard of MRSA CI2 growth inhibition with varying concentrations of PNPs and AS. ( D ) Schematic checkerboard of MRSA CI3 growth inhibition with varying concentrations of PNPs and AS.

Article Snippet: Next, 100 μL of the MRSA bacterial suspensions (ATCC 43300, ATCC 33591, CI2, and CI3) was exposed to equal volumes of PNPs, AS, and PNPs in combination with AS.

Techniques: Inhibition

Journal: Microorganisms

Article Title: Antimicrobial Activities of Propolis Nanoparticles in Combination with Ampicillin Sodium Against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/microorganisms13081844

Figure Lengend Snippet: Effects of different samples on MRSA biofilm formation: ( A ) 16 μg/mL of AS, 32 μg/mL of PNPs, and the combination AS + PNPs against MRSA ATCC 43300; ( B ) 8 μg/mL of AS, 32 μg/mL of PNPs, and the combination AS + PNPs against MRSA ATCC 33591; ( C ) 32 μg/mL of AS, 64 μg/mL of PNPs, and the combination AS + PNPs against MRSA CI2; ( D ) 32 μg/mL of AS, 32 μg/mL of PNPs, and the combination AS + PNPs against MRSA CI3 (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Next, 100 μL of the MRSA bacterial suspensions (ATCC 43300, ATCC 33591, CI2, and CI3) was exposed to equal volumes of PNPs, AS, and PNPs in combination with AS.

Techniques:

Journal: Microorganisms

Article Title: Antimicrobial Activities of Propolis Nanoparticles in Combination with Ampicillin Sodium Against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/microorganisms13081844

Figure Lengend Snippet: Effects of different samples on mature MRSA biofilm: ( A ) 16 μg/mL of AS, 32 μg/mL of PNPs, and the combination AS + PNPs against MRSA ATCC 43300; ( B ) 8 μg/mL of AS, 32 μg/mL of PNPs, and the combination AS + PNPs against MRSA ATCC 33591; ( C ) 32 μg/mL of AS, 64 μg/mL of PNPs, and the combination AS + PNPs against MRSA CI2; ( D ) 32 μg/mL of AS, 32 μg/mL of PNPs, and the combination AS + PNPs against MRSA CI3 (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Next, 100 μL of the MRSA bacterial suspensions (ATCC 43300, ATCC 33591, CI2, and CI3) was exposed to equal volumes of PNPs, AS, and PNPs in combination with AS.

Techniques:

Journal: Microorganisms

Article Title: Antimicrobial Activities of Propolis Nanoparticles in Combination with Ampicillin Sodium Against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/microorganisms13081844

Figure Lengend Snippet: Effects of different concentrations of PNPs on bacterial adhesion and extracellular polysaccharide production of MRSA ATCC 43300. ( A ) PNPs inhibit bacterial adhesion; ( B ) PNPs inhibit alkali-soluble polysaccharide production; ( C ) PNPs inhibit water-soluble polysaccharide production (*** p < 0.001).

Article Snippet: Next, 100 μL of the MRSA bacterial suspensions (ATCC 43300, ATCC 33591, CI2, and CI3) was exposed to equal volumes of PNPs, AS, and PNPs in combination with AS.

Techniques:

Journal: Microorganisms

Article Title: Antimicrobial Activities of Propolis Nanoparticles in Combination with Ampicillin Sodium Against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/microorganisms13081844

Figure Lengend Snippet: Effects of PNPs on the expression of the mecA , mecR1 , and blaZ genes of MRSA ATCC 43300. ( A ) PNPs inhibit the expression of mecA ; ( B ) PNPs inhibit the expression of mecR1 ; ( C ) PNPs inhibit the expression of blaZ (*** p < 0.001).

Article Snippet: Next, 100 μL of the MRSA bacterial suspensions (ATCC 43300, ATCC 33591, CI2, and CI3) was exposed to equal volumes of PNPs, AS, and PNPs in combination with AS.

Techniques: Expressing

Journal: Microorganisms

Article Title: Antimicrobial Activities of Propolis Nanoparticles in Combination with Ampicillin Sodium Against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/microorganisms13081844

Figure Lengend Snippet: Effects of PNPs on the expression of the icaADBC genes of MRSA ATCC 43300. ( A ) PNPs inhibit the expression of icaA ; ( B ) PNPs inhibit the expression of icaD ; ( C ) PNPs inhibit the expression of icaB ; ( D ) PNPs inhibit the expression of icaC (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Next, 100 μL of the MRSA bacterial suspensions (ATCC 43300, ATCC 33591, CI2, and CI3) was exposed to equal volumes of PNPs, AS, and PNPs in combination with AS.

Techniques: Expressing